GLP-1 peptide reference
Semaglutide: Reconstitution Math & Reference
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How do you reconstitute semaglutide?
Semaglutide reconstitution is arithmetic: divide vial mass (mg) by added bacteriostatic water (ml) to get mg/ml. A 5 mg vial in 2.5 ml BAC water gives 2 mg/ml — so a 0.25 mg target dose is 0.125 ml, or 12.5 units on a U-100 syringe. Peptly computes this instantly and shows the exact mark on a visual syringe.
What is Semaglutide?
Semaglutide is a 31-amino-acid GLP-1 receptor agonist. It is the active ingredient in branded products including Ozempic and Wegovy.
Semaglutide is FDA-approved as a prescription medication under brand names including Ozempic (type-2 diabetes) and Wegovy (chronic weight management). Peptly is a calculator for research and reference, not a prescription substitute.
Compounded or research-grade semaglutide commonly ships as lyophilized powder in vials of 2 mg, 3 mg, 5 mg, or 10 mg. Reconstitution math follows the standard vial-mass-over-water-volume formula.
Because semaglutide doses are typically much smaller than research peptides like BPC-157 (often 0.25 mg to 2.4 mg per dose), unit counts on a U-100 syringe are small. Peptly's visual syringe view makes the exact draw mark unmistakable.
Reconstitution math — reference table
| Vial | BAC water | Concentration | Example dose | U-100 units |
|---|---|---|---|---|
| 2 mg | 1 ml | 2 mg/ml | 0.25 mg | 12.5 units |
| 5 mg | 2.5 ml | 2 mg/ml | 0.5 mg | 25 units |
| 5 mg | 5 ml | 1 mg/ml | 0.5 mg | 50 units |
| 10 mg | 4 ml | 2.5 mg/ml | 1 mg | 40 units |
| 10 mg | 5 ml | 2 mg/ml | 1 mg | 50 units |
Storage and shelf-life
Reconstituted semaglutide is most commonly refrigerated at 2–8 °C and used within 4–6 weeks per common research protocols. Lyophilized powder typically stores at −20 °C until reconstitution.
How Peptly handles Semaglutide
- Pre-loaded compound profile — no manual entry of vial mass each time.
- Saved reconstitution mixes — reload any combination in one tap.
- Visual U-100 syringe view — see the draw mark before you pull it.
- Injection log — every shot, site, and cycle stored on-device.
- Smart reminders — schedule-aware notifications with site rotation prompts.
Other peptide references
- BPC-157 reconstitution math — BPC-157 is a synthetic pentadecapeptide — a 15-amino-acid fragment derived from a protective protein originally isolated from human gastric juice.
- TB-500 reconstitution math — TB-500 is a 17-amino-acid synthetic peptide fragment of Thymosin Beta-4, a regulatory peptide naturally present in most mammalian cells.
- Tirzepatide reconstitution math — Tirzepatide is a dual GIP/GLP-1 receptor agonist — a 39-amino-acid synthetic peptide.
- Ipamorelin reconstitution math — Ipamorelin is a 5-amino-acid synthetic peptide and a selective growth hormone secretagogue.
Related tools and guides
- Main peptide calculator overview
- Peptide reconstitution math reference
- BAC water calculator
- Step-by-step reconstitution guide
- Peptide dosage unit reference
- Peptide tracker — injection log
Frequently asked questions
How is reconstitution math different for semaglutide vs research peptides like BPC-157? +
The arithmetic is identical — concentration equals vial mass divided by BAC water volume. The only practical difference is that semaglutide doses are typically much smaller (0.25 mg to 2.4 mg), so unit counts on a U-100 syringe land in the 10-50 unit range.
What BAC water volume works best for a 5 mg semaglutide vial? +
Common research protocols pick a BAC volume so that a single dose lands between 10–50 units on a U-100 syringe — readable without micropipetting. For a 5 mg vial, 2 ml or 2.5 ml are common starting points. Peptly shows the unit count for any combination instantly.
Does Peptly distinguish between Ozempic, Wegovy, and compounded semaglutide? +
Peptly tracks semaglutide as a single compound profile because the molecule is the same. Branded pens like Ozempic come pre-mixed at a fixed concentration — the calculator is mostly used for compounded or research-grade lyophilized vials.
Can the injection log handle a tapered titration schedule? +
Yes. In Peptly Pro, scheduled doses can change over time (e.g. 0.25 mg → 0.5 mg → 1 mg). The log records the actual dose drawn against the saved reconstitution mix.
Is semaglutide approved for human use? +
Yes — semaglutide is FDA-approved under brand names including Ozempic and Wegovy. Compounded or research-grade semaglutide is a different supply category. Peptly is a calculator and reference, not a prescription substitute. Consult a licensed clinician.