GLP-1 peptide reference
Semaglutide: Reconstitution Math & Reference
By Baris Bingor · Last updated
How do you reconstitute semaglutide?
Semaglutide reconstitution is arithmetic: divide vial mass (mg) by added bacteriostatic water (ml) to get mg/ml. A 5 mg vial in 2.5 ml BAC water gives 2 mg/ml — so a 0.25 mg target dose is 0.125 ml, or 12.5 units on a U-100 syringe. The calculator below does it for any numbers you type and draws the line on a syringe.
Reconstitution calculator
Type your own numbers. They aren't sent anywhere: the math runs in your browser.
Type an amount to see the volume and the units.
Arithmetic only. Peptly does not suggest amounts: the amount is yours, from your protocol or the product's documentation. Not medical advice.
What is Semaglutide?
Semaglutide is a 31-amino-acid GLP-1 receptor agonist. It is the active ingredient in branded products including Ozempic and Wegovy.
Semaglutide is FDA-approved as a prescription medication under brand names including Ozempic (type-2 diabetes) and Wegovy (chronic weight management). This page is a math reference for research, not a prescription substitute.
Compounded or research-grade semaglutide commonly ships as lyophilized powder in vials of 2 mg, 3 mg, 5 mg, or 10 mg. Reconstitution math follows the standard vial-mass-over-water-volume formula.
Because semaglutide doses are typically much smaller than research peptides like BPC-157 (often 0.25 mg to 2.4 mg per dose), unit counts on a U-100 syringe are small. The calculator above draws the line on a syringe, so a small draw is easy to picture.
Reconstitution math — reference table
| Vial | BAC water | Concentration | Example dose | U-100 units |
|---|---|---|---|---|
| 2 mg | 1 ml | 2 mg/ml | 0.25 mg | 12.5 units |
| 5 mg | 2.5 ml | 2 mg/ml | 0.5 mg | 25 units |
| 5 mg | 5 ml | 1 mg/ml | 0.5 mg | 50 units |
| 10 mg | 4 ml | 2.5 mg/ml | 1 mg | 40 units |
| 10 mg | 5 ml | 2 mg/ml | 1 mg | 50 units |
Storage and shelf-life
Reconstituted semaglutide is most commonly refrigerated at 2–8 °C and used within 4–6 weeks per common research protocols. Lyophilized powder typically stores at −20 °C until reconstitution.
Semaglutide in the Peptly app
- A plain-language profile in the library — no amounts, frequencies or regimens.
- Entries exactly as you type them — the app never calculates, converts or suggests an amount.
- Today and reminders — log or skip each entry in one tap, on the schedule you set.
- Injection-site rotation — Peptly suggests the site you used longest ago, other than the last one.
- Private by design — no account; your log stays on your phone.
Other peptide references
- BPC-157 reconstitution math — BPC-157 is a synthetic pentadecapeptide — a 15-amino-acid fragment derived from a protective protein originally isolated from human gastric juice.
- TB-500 reconstitution math — TB-500 is a 17-amino-acid synthetic peptide fragment of Thymosin Beta-4, a regulatory peptide naturally present in most mammalian cells.
- Tirzepatide reconstitution math — Tirzepatide is a dual GIP/GLP-1 receptor agonist — a 39-amino-acid synthetic peptide.
- Ipamorelin reconstitution math — Ipamorelin is a 5-amino-acid synthetic peptide and a selective growth hormone secretagogue.
- CJC-1295 reconstitution math — CJC-1295 is a synthetic 30-amino-acid analog of growth hormone-releasing hormone (GHRH 1-29), modified for protease resistance.
- Retatrutide reconstitution math — Retatrutide (LY3437943) is an investigational triple-hormone-receptor agonist — it targets the GLP-1, GIP, AND glucagon receptors.
- Tesamorelin reconstitution math — Tesamorelin is a synthetic 44-amino-acid analog of human growth hormone-releasing hormone (GHRH 1-44), stabilized with a trans-3-hexenoic acid group.
- GHK-Cu reconstitution math — GHK-Cu is a copper complex of the naturally occurring tripeptide glycyl-L-histidyl-L-lysine (Gly-His-Lys), which binds a single copper(II) ion.
Related tools and guides
- Main peptide calculator overview
- Peptide reconstitution math reference
- BAC water calculator
- Step-by-step reconstitution guide
- Peptide storage & refrigeration guide
- Peptide dosage unit reference
- Peptide tracker — injection log
Frequently asked questions
How is reconstitution math different for semaglutide vs research peptides like BPC-157? +
The arithmetic is identical — concentration equals vial mass divided by BAC water volume. The only practical difference is that semaglutide doses are typically much smaller (0.25 mg to 2.4 mg), so unit counts on a U-100 syringe land in the 10-50 unit range.
What BAC water volume works best for a 5 mg semaglutide vial? +
Common research protocols pick a BAC volume so that a single dose lands between 10–50 units on a U-100 syringe — readable without micropipetting. For a 5 mg vial, 2 ml or 2.5 ml are common starting points. The calculator on this page shows the unit count for any combination as you type.
Does Peptly distinguish between Ozempic, Wegovy, and compounded semaglutide? +
No: the Peptly app's library has one semaglutide profile, because the molecule is the same. Branded pens like Ozempic come pre-mixed at a fixed concentration — reconstitution math, like the calculator on this page, is for compounded or research-grade lyophilized vials.
Can the injection log follow a titration schedule? +
It records one, but never sets it. Peptly never suggests a titration step or an amount: when your amount changes, you enter the new one, and each entry keeps the amount exactly as you typed it, so your history shows every step.
Is semaglutide approved for human use? +
Yes — semaglutide is FDA-approved under brand names including Ozempic and Wegovy. Compounded or research-grade semaglutide is a different supply category. This page is a math reference and the Peptly app is a log — neither is a prescription substitute. Consult a licensed clinician.