Peptide reference
TB-500: Reconstitution Math & Reference
By Baris Bingor · Last updated
How do you reconstitute TB-500?
TB-500 reconstitution math is identical to other lyophilized peptides: divide vial mass (mg) by added bacteriostatic water (ml) to get mg/ml. A 5 mg vial with 2 ml BAC water yields 2.5 mg/ml. Drawing 20 units on a U-100 syringe (0.2 ml) then delivers 500 mcg. The calculator below does it for any numbers you type.
Reconstitution calculator
Type your own numbers. They aren't sent anywhere: the math runs in your browser.
Type an amount to see the volume and the units.
Arithmetic only. Peptly does not suggest amounts: the amount is yours, from your protocol or the product's documentation. Not medical advice.
What is TB-500?
TB-500 is a 17-amino-acid synthetic peptide fragment of Thymosin Beta-4, a regulatory peptide naturally present in most mammalian cells.
TB-500 has been studied in animal models exploring cell migration, angiogenesis, and tissue regeneration. It is not FDA-approved for human use.
TB-500 commonly ships in 2 mg, 5 mg, or 10 mg lyophilized vials. Reconstitution math follows the same vial-mass-over-water-volume formula as every other peptide.
Reconstitution math — reference table
| Vial | BAC water | Concentration | Example dose | U-100 units |
|---|---|---|---|---|
| 2 mg | 1 ml | 2 mg/ml | 500 mcg | 25 units |
| 5 mg | 2 ml | 2.5 mg/ml | 500 mcg | 20 units |
| 5 mg | 3 ml | 1.67 mg/ml | 500 mcg | 30 units |
| 10 mg | 3 ml | 3.33 mg/ml | 1 mg | 30 units |
| 10 mg | 5 ml | 2 mg/ml | 1 mg | 50 units |
Storage and shelf-life
Most research protocols refrigerate reconstituted TB-500 at 2–8 °C and use within 2–4 weeks. Lyophilized powder typically stores at −20 °C.
TB-500 in the Peptly app
- A plain-language profile in the library — no amounts, frequencies or regimens.
- Entries exactly as you type them — the app never calculates, converts or suggests an amount.
- Today and reminders — log or skip each entry in one tap, on the schedule you set.
- Injection-site rotation — Peptly suggests the site you used longest ago, other than the last one.
- Private by design — no account; your log stays on your phone.
Other peptide references
- BPC-157 reconstitution math — BPC-157 is a synthetic pentadecapeptide — a 15-amino-acid fragment derived from a protective protein originally isolated from human gastric juice.
- Semaglutide reconstitution math — Semaglutide is a 31-amino-acid GLP-1 receptor agonist.
- Tirzepatide reconstitution math — Tirzepatide is a dual GIP/GLP-1 receptor agonist — a 39-amino-acid synthetic peptide.
- Ipamorelin reconstitution math — Ipamorelin is a 5-amino-acid synthetic peptide and a selective growth hormone secretagogue.
- CJC-1295 reconstitution math — CJC-1295 is a synthetic 30-amino-acid analog of growth hormone-releasing hormone (GHRH 1-29), modified for protease resistance.
- Retatrutide reconstitution math — Retatrutide (LY3437943) is an investigational triple-hormone-receptor agonist — it targets the GLP-1, GIP, AND glucagon receptors.
- Tesamorelin reconstitution math — Tesamorelin is a synthetic 44-amino-acid analog of human growth hormone-releasing hormone (GHRH 1-44), stabilized with a trans-3-hexenoic acid group.
- GHK-Cu reconstitution math — GHK-Cu is a copper complex of the naturally occurring tripeptide glycyl-L-histidyl-L-lysine (Gly-His-Lys), which binds a single copper(II) ion.
Related tools and guides
- Main peptide calculator overview
- Peptide reconstitution math reference
- BAC water calculator
- Step-by-step reconstitution guide
- Peptide storage & refrigeration guide
- Peptide dosage unit reference
- Peptide tracker — injection log
Frequently asked questions
How is TB-500 reconstitution different from Thymosin Beta-4? +
TB-500 is a 17-amino-acid fragment of Thymosin Beta-4 (which is 43 amino acids long). Reconstitution arithmetic is identical — both are dissolved in bacteriostatic water based on vial mass.
What concentration do most TB-500 research protocols use? +
Research protocols commonly target 2–5 mg/ml so that typical mass doses (500 mcg to 2 mg) draw between 10–50 units on a U-100 syringe — readable on a standard insulin scale.
Can Peptly log TB-500 and BPC-157 separately? +
Yes. Each entry records the peptide it is for, so your history keeps them apart. Logging more than one peptide (stacks) is part of Peptly Pro; the free version covers one peptide.
Why does the unit count change when I switch BAC water from 2 ml to 3 ml? +
More water dilutes the same vial mass to a lower mg/ml concentration. Lower concentration means more volume (more units) is needed for the same mass dose. The calculator on this page recomputes it as you change the water volume.
Is TB-500 approved for human use? +
No. TB-500 is not FDA-approved for human use. This page is a math reference and the Peptly app is a log — neither is medical advice.