BPC-157 Dosing Guide: Research-Framed Reference

How do you calculate a BPC-157 dose?

BPC-157 dose math has three steps. (1) Concentration (mg/ml) = vial mass ÷ BAC water volume. (2) Draw volume (ml) = target dose ÷ concentration. (3) U-100 units = draw volume × 100. Example: 5 mg vial + 1 ml BAC = 5 mg/ml; a 250 mcg dose = 0.25 mg ÷ 5 = 0.05 ml = 5 units. The free calculator on this site does it for any numbers you type.

What you need

• Lyophilized BPC-157 vial (5 mg or 10 mg)
• Bacteriostatic water for injection (BWFI)
• U-100 insulin syringe (standard for subq research dosing)
• Isopropyl alcohol swabs
• A calculator for the math — the free one on this site runs in your browser

The reconstitution math

Three formulas, same as any peptide:

• Concentration (mg/ml) = vial mass (mg) ÷ BAC water (ml). Example: 5 mg ÷ 1 ml = 5 mg/ml.
• Draw volume (ml) = target dose (mg) ÷ concentration (mg/ml). Example: 0.25 mg ÷ 5 mg/ml = 0.05 ml.
• U-100 units = draw volume × 100. Example: 0.05 × 100 = 5 units.

The key choice is BAC water volume. For BPC-157's small doses (250-500 mcg), a higher BAC volume (= lower concentration) spreads the dose across more readable units. A 5 mg vial + 2 ml BAC gives 2.5 mg/ml, where 250 mcg = 10 units (easier to read) and 500 mcg = 20 units.

Reference protocols

1. Common research dose range. Most published research protocols use 250-500 mcg per dose, once or twice daily, for 4-8 weeks. Some protocols go up to 1000 mcg/day total. Always defer to the specific study you are following.

2. Cadence. Once daily (e.g., morning) for general protocols. Twice daily (morning + evening) for some targeted-injury protocols. Cycle length 4-8 weeks, then a 2-4 week off-period before another cycle.

3. Subcutaneous vs intramuscular. Most research uses subcutaneous (subq) injection — abdomen quadrants. Some protocols use intramuscular (IM) near a targeted tissue region.

4. Vial size on the research market. Common vial sizes: 5 mg or 10 mg lyophilized. A 5 mg vial at 250 mcg/dose lasts 20 doses; at 500 mcg/dose, 10 doses. A 10 mg vial doubles that. Match vial size to your cycle length to minimize storage time after reconstitution.

BPC-157 dose math quick reference

Vial + BAC	Concentration	250 mcg dose	500 mcg dose
5 mg + 1 ml	5 mg/ml	0.05 ml = 5 units	0.1 ml = 10 units
5 mg + 2 ml	2.5 mg/ml	0.1 ml = 10 units	0.2 ml = 20 units
10 mg + 2 ml	5 mg/ml	0.05 ml = 5 units	0.1 ml = 10 units
10 mg + 3 ml	3.33 mg/ml	0.075 ml ≈ 7.5 units	0.15 ml = 15 units

Round to the nearest unit you can read on the syringe. The calculator on this site shows the units to one decimal place, so you can see the margin before you round.

Step-by-step

1. Confirm vial mass. Read the label. Most BPC-157 research vials are 5 mg or 10 mg lyophilized powder. Note the exact mass — the math depends on it.

2. Pick a BAC water volume. For 5 mg vial: 1 ml BAC gives 5 mg/ml (250 mcg = 5 units, 500 mcg = 10 units). For 10 mg vial: 2 ml BAC gives 5 mg/ml (same per-dose math). Higher BAC volumes shift the unit count larger; pick the volume that lands your typical dose at 5-20 units on a U-100 syringe.

3. Reconstitute slowly. Wipe the vial stopper with isopropyl. Inject BAC water down the inside wall of the vial — not directly at the powder. Swirl gently until dissolved. Do not shake.

4. Work out the draw. Divide the vial mass by the BAC water to get mg/ml, divide the amount by it, and multiply by 100 for U-100 units. The free calculator on this site does it for the numbers you type and draws the line on a syringe.

5. Write it down. Note the date, the water added and the concentration on the vial label, so later draws from the same vial do not need the math again.

Common mistakes

• Mixing up mg and mcg. 1 mg = 1,000 mcg. A 0.25 mg dose is 250 mcg. Most BPC-157 protocols quote doses in mcg, so the conversion gets easy to mis-read.
• Reading U-40 units on a U-100 syringe. Confirm the syringe label says U-100 before drawing.
• Choosing too little BAC water. A 5 mg + 0.5 ml mix is 10 mg/ml, where 250 mcg is 2.5 units — hard to read on a 100-unit syringe. Use more BAC for sharper unit counts.
• Skipping the log. Without a tracker, dose #14 of a 28-day cycle is hard to verify. Peptly's log keeps every entry with its site, date and time.
• Hot-spotting one injection site. Repeated injections in the same site can cause local tissue stress. Rotate quadrants; Peptly's log suggests your next site.

How a tracked log helps

• Today and reminders. See what's planned and log or skip each entry in one tap; reminders follow your own schedule.
• Site rotation. Peptly suggests your next site: the one you used longest ago, other than the last one.
• Cycle awareness. Day 1 of a 6-week cycle is different from day 35. Each entry keeps its date, so the timeline is on the record (the last 30 days free, all of it with Pro).
• CSV export (Pro). Export your full history when you want a copy for your records.
• On-device log. No accounts and no cloud sync. The log lives on your phone.

See also

• Peptide calculator
• Peptide injection tracker
• GLP-1 dose calculator — Semaglutide, Tirzepatide
• Tirzepatide vs Semaglutide
• How to reconstitute peptides — step-by-step
• BAC water calculator
• Peptide dosage reference

Primary sources & evidence status

BPC-157 evidence is preclinical. It was first described by the team of Predrag Sikirić (University of Zagreb) in the 1990s and has been studied in 100+ animal models. As of 2024–2025 systematic reviews there are no completed controlled human efficacy trials and no human clinical safety data, and reported plasma half-life is under 30 minutes. Every figure on this page is a reconstitution-math reference for research — not clinical guidance, and not a recommendation for human use.

• Stable Gastric Pentadecapeptide BPC 157 and Wound Healing — review, Front. Pharmacol., 2021 (preclinical / animal models).
• The Stable Gastric Pentadecapeptide BPC 157: Pleiotropic Beneficial Activity — review, 2024 (preclinical / animal models).
• BPC 157 and Intestinal Anastomoses Therapy in Rats — A Review — PMC, 2024 (animal model).

Frequently asked questions

What is the standard dose of BPC-157? +

There is no FDA-approved dose because BPC-157 is not an FDA-approved drug. Published research protocols typically use 250-500 mcg per dose, once or twice daily, for 4-8 weeks. Some protocols go higher. Always defer to the specific research literature you are following — Peptly does not recommend doses.

How many units of BPC-157 is 250 mcg on a U-100 syringe? +

Depends on your concentration. At 5 mg/ml (5 mg vial + 1 ml BAC), 250 mcg = 0.05 ml = 5 units. At 10 mg/ml (5 mg vial + 0.5 ml BAC), 250 mcg = 0.025 ml = 2.5 units. At 2.5 mg/ml (5 mg vial + 2 ml BAC), 250 mcg = 0.1 ml = 10 units. The free calculator on this site does it for any numbers you type.

Subcutaneous or intramuscular for BPC-157? +

Most published protocols use subcutaneous injection. Some targeted-injury protocols use intramuscular near a specific tissue region. The reconstitution math is the same — only the injection site and needle length differ. Subq uses a short insulin needle; IM needs a longer needle (1 inch or more).

How long is reconstituted BPC-157 stable? +

Most research protocols refrigerate reconstituted BPC-157 at 2-8 °C and use within 4-6 weeks when reconstituted with bacteriostatic water. Stability varies by source peptide and storage conditions. Some sources cite shorter windows. Consult primary literature for your specific peptide.

Can I rotate injection sites for BPC-157? +

Yes. For subcutaneous BPC-157, standard rotation across four abdomen quadrants is the common pattern. Some targeted-injury protocols inject near the affected tissue, in which case rotation is limited but still recommended within the immediate area. Peptly's log suggests your next site: the one you used longest ago, other than the last one.

Does the Peptly app work out BPC-157 doses? +

No. The Peptly app is a log, not a calculator: it keeps each entry with the amount exactly as you typed it, the site and the date, reminds you on your own schedule and suggests your next site. It never calculates, suggests or recommends an amount. For the arithmetic, the free calculator on this site works in your browser.

What about TB-500 stacking? +

Some research protocols stack BPC-157 with TB-500 (Thymosin Beta-4 fragment) on alternating days or in the same injection. The reconstitution math is independent — each peptide gets its own vial + BAC water + concentration. In the Peptly app, logging more than one peptide (stacks) is part of Peptly Pro, with a schedule and reminders for each.

Does Peptly plan BPC-157 cycles? +

No. Peptly never recommends a cycle length or an amount. You set your own schedule, Peptly reminds you when each entry is due, and your history shows the timeline (the last 30 days free, all of it with Peptly Pro).